B100 Biodiesel: Pure Biodiesel for Approved Engines

B100 is 100% biodiesel — no petroleum diesel mixed in. It's the highest-emissions-reduction option but also the highest-touch fuel: limited engine compatibility, cold-weather sensitivity, shorter storage windows, and almost no presence at retail. Most B100 in the US ends up in dedicated fleet operations rather than passenger vehicles.

Last reviewed: April 29, 2026

ASTM D6751 — B100 spec snapshot

  • StandardASTM D6751
  • Flash point (min)93°C
  • Sulfur, S15 grade15 ppm
  • Sulfur, S500 grade500 ppm
  • Cetane number (min)47
  • Oxidation stability (min)3 hr Rancimat
  • Free glycerin (max)0.020% mass
  • Total glycerin (max)0.240% mass
  • Water & sediment (max)0.050% vol
  • Cloud pointreported

Energy content

  • B100 LHV~119,550 BTU/gal
  • vs petroleum diesel−7%

OEM B100 approval status

  • Ford Power Strokeno
  • Cummins on-highway (stock)no — B20 max
  • GM Duramax (current)no — B20 max
  • Detroit Diesel DD13/15/16no — B20 max
  • Caterpillar on-highwayno — B20 max
  • Navistar Internationalno — B20 max
  • John Deere Tier 4 ag (select)approved
  • Optimus-converted Cummins/DDapproved (aftermarket)
  • Stationary engines / gensetsmodel-specific
  • Pre-2007 IDI Mercedes / VWhistorically OK, no warranty

Lifecycle GHG (vs petroleum diesel)

  • Used cooking oil / tallow−70 to −86%
  • Soy / canola (with LUC)−40 to −69%

Source: ASTM D6751 standard summary via AFDC; OEM positions from current Cummins Service Bulletin 3379001, Ford Power Stroke Owner's Manuals, GM Duramax service guides, John Deere Tier 4 fluid recommendations, and Optimus Technologies B100 Vector System documentation. Lifecycle GHG ranges from Argonne National Laboratory's GREET model (current release).

Before running B100: confirm your engine manufacturer specifically approves B100 for your engine family. Most on-highway diesel OEMs cap warranty coverage at B20. Running B100 in a non-approved engine can void the fuel-system warranty and may damage seals, hoses, or injectors over time.

What B100 is, technically

B100 is a fuel produced from vegetable oils, animal fats, or waste cooking oil through a chemical process called transesterification. The triglyceride molecules in the feedstock react with methanol to form fatty-acid methyl esters (FAME, the actual biodiesel) and glycerin (a byproduct). What gets sold as B100 is purified FAME meeting ASTM D6751.

D6751 specifies four grades:

Spec parameters that matter most for end users:

ParameterD6751 requirementWhy it matters
Flash point93°C minimumStorage and transport safety; about 35°C higher than petroleum diesel
Sulfur15 ppm (S15) or 500 ppm (S500)S15 grade is required for use in 2007+ on-highway engines with diesel particulate filters
Cloud pointReported (no max specified)Determines cold-weather operability for the specific batch
Oxidation stability3 hours minimum (Rancimat)Determines safe storage life; higher numbers mean longer shelf life
Free glycerin0.020% mass maxExcess glycerin causes injector deposits and filter plugging
Water and sediment0.050% volume maxWater supports microbial growth and corrodes fuel systems

Source: ASTM D6751 standard summary via AFDC. The full standard is available from ASTM International for purchase.

Engine compatibility — narrower than B20

This is the part that disqualifies most users. Major on-highway OEMs cap B-level approval at B20 for standard production engines:

B100 approvals exist primarily in:

If you have a specific engine and you want to run B100, the right move is to request the OEM's current biodiesel position statement in writing, then verify the specific engine serial number is covered.

Energy content and fuel economy

B100 carries about 119,550 BTU per gallon, compared to roughly 128,488 for petroleum diesel — about 7% lower. This translates to a real-world fuel-economy drop of about 7% in vehicles approved to run it.

For a fleet, this means a vehicle that gets 8 mpg on petroleum diesel will get about 7.4 mpg on B100. The economics depend on the price per gallon spread (often favorable for B100 when biodiesel-tax-credit benefits flow through) and the value of the GHG reduction (LCFS credits in California, RFS RIN values nationally).

Cold-weather operation

This is B100's biggest practical limitation. The cloud point of B100 is typically 30-40°F, depending on feedstock — soybean B100 clouds around 32°F; tallow-derived B100 can cloud as high as 60°F. Below the cloud point, wax crystals form and start plugging filters. Below the pour point, the fuel won't flow at all.

Cold-weather management options for B100 fleets:

Storage and shelf life

B100 oxidizes faster than petroleum diesel. Without antioxidant additives, B100 oxidation stability ranges from a few months to about 12 months depending on feedstock and storage conditions. With antioxidants, 12-24 months is achievable.

Practical storage rules for bulk B100:

Where B100 is sold

Retail B100 is rare. The AFDC station locator does include the small number of public B100 stations alongside B20 stations, but most US B100 volume moves through:

If you're a small fleet or an individual looking to source B100, your most likely path is contacting a regional biodiesel producer directly. Clean Fuels Alliance America publishes a member directory; state-level renewable fuels associations (Iowa RFA, Minnesota Biofuels Association, others) maintain similar lists.

For everyday purchases of biodiesel, B20 is the more practical option for most operators. Use the station locator to find B20 stations near you.

All blends compared →

B5, B20, B100 side-by-side.

B5

5% biodiesel — the unlabeled blend in most U.S. diesel pumps.

B20

The most common labeled blend at U.S. retail.